SEROTYPING OF HUMAN ROTAVIRUS BY ENZYME IMMUNO-ASSAY WITH MONOCLONAL ANTIBODIES
نویسندگان
چکیده
منابع مشابه
Serotyping of human rotavirus by enzyme immuno-assay with monoclonal antibodies.
Two series of enzyme immunoassays with monoclonal antibodies produced in two laboratories (A and B) were compared in use for serotyping of human rotavirus in stool samples collected in Japan between 1988 and 1991 from patients with gastroenteritis. Of 358 samples, 222 were determined to be the same serotype, while 61 samples could not be serotyped by either assay. A hundred and one and 92 sampl...
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Ureaplasma urealyticum comprises 14 serotypes. The existing serotyping methods all use polyclonal antibodies. These methods are time-consuming and labor-intensive, and they cannot always be performed on primary isolates; in addition, the results are difficult to interpret. We developed a new enzyme-linked immunosorbent assay (ELISA) method using serotype-specific monoclonal antibodies (MAbs) to...
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Three monoclonal antibodies (MAbs)--B5C9, B5E4, and B10G10--to human group B rotavirus, an agent implicated in epidemic outbreaks of diarrhea in the People's Republic of China, primarily in adults, were prepared. MAb reactivity was decreased when virus preparations were treated with EDTA, suggesting reactivity with the outer-capsid protein(s). Competition experiments suggested that these MAbs r...
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A human antibody library constructed by utilizing a phage display system was used for the isolation of human antibodies with neutralizing activity specific for human rotavirus. In the library, the Fab form of an antibody fused to truncated cp3 is expressed on the phage surface. Purified virions of strain KU (G1 serotype and P[8] genotype) were used as antigen. Twelve different clones were isola...
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Monoclonal antibodies specific for the schizont and merozoite stages of Plasmodium falciparum have been used to demonstrate antigenic differences between parasites obtained from individual patients with acute malaria. Parasites from East Africa are shown to share some strain-specific antigenic determinants with culture-adapted isolates from Africa as well as from Asia and Papua New Guinea.
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ژورنال
عنوان ژورنال: Japanese Journal of Medical Science and Biology
سال: 1993
ISSN: 0021-5112,1884-2828
DOI: 10.7883/yoken1952.46.221